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Doris Sanders. For more information on a book by a favorite author, just check out this free book summary and reviews. When I learned of this new book, I was very excited to hear that it contained the. A book that is insightful and gives you a good idea of what it is like to. Title: A Passion for Fashion, Desire for Style: The Authors of DK Living Books, ISBN: 978-1453905789,. the sample, and rates of return were calculated as described previously \[[@B8]\].
Additional genes and primers for qPCR
-------------------------------------
The primers used for qPCR analysis were designed with the aid of the Primer Express^R^3.0 software (Applied Biosystems, Foster City, CA, USA), using default parameters, and using sequences identified in *Brucella*species deposited in GenBank ( Gene names are listed in the Tables [2](#T2){ref-type="table"} and [3](#T3){ref-type="table"}. Quantification of 16S rRNA was performed with primer Q-16S3R, which corresponds to the sequence of the conserved region of 16S rRNA of *B. melitensis*strain M28 \[[@B27]\]. The procedure was performed with the Light Cycler^R^System 480 (Roche) as described elsewhere \[[@B28]\]. Expression of *aceA*(BMEII0627), *acpP*(BMEI0871), *bfmB*(BMEI0812), *bfrB*(BMEII1332), *bvrR*(BMEII0813), *cysC*(BMEI0107), *narK*(BMEI1075), *narK2*(BMEI0999), *narL*(BMEII0865), *narP*(BMEII1042), *narU*(BMEI0308), * 0b46394aab
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